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KMID : 0358920190460010085
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Journal of the Korean Academy of Pedodontics
2019 Volume.46 No. 1 p.85 ~ p.92
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Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth
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Yun Ji-Hye
You Yong-Ouk
Ahn Eun-Suk
Lee Jun
An So-Youn
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Abstract
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The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro MTA® (RM), EZ-SealTM (EZ) and ENDOCEM Zr® (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ p( = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.
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KEYWORD
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Cytotoxicity, Calcium silicate, Mineral trioxide aggregate, Deciduous tooth, Stem cells
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